Virus:Article Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Bacteroides thetaiotaomicron VPI-5482 ATCC ATCC 29148 Bacteroides thetaiotaomicron VPI-5482 pNBU2 This work N/A Bacteroides thetaiotaomicron VPI-5482 pNBU2::trpD This work N/A Chemicals, Peptides, and Recombinant Proteins Ca2+ Mg-free PBS Corning 21-040-CV Gentle cell dissociation media STEMCELL Technologies Cat# 7174 Bovine serum albumin Sigma Life Science Cas# 9048-46-8 DMEM-F/12 media Life Technologies Cat# 11330-032 IntestiCultTM organoid growth media STEMCELL Technologies Cat# 6000 Matrigel Corning Cat# 356231 Calcein green Invitrogen Cat# C3100MP Tryptamine hydrochloride Aldrich Cas# 343-92-2 GR-113808 Tocris Bioscience Cas# 144625-51-4 Ondansetron Sigma-Aldrich Cas# 99614-01-04 Forskolin Sigma-Aldrich Cas# 66575-29-9 Erythromycin Sigma Life Science Cas# 114-07-8 Gentamycin Sigma Life Science Cas# 1405-41-0 Methanol Sigma-Aldrich Cas# 67-56-1 Methylcellulose Sigma-Aldrich Cas#9004-67-5 Carmine red Sigma-Aldrich Cas# 1390-65-4 5-Nitro-2-(3-phenylpropylamino) benzoic acid Sigma-Aldrich Cas# 107254-86-4 Amiloride hydrochloride Sigma-Aldrich Cas# 2016-88-8 CH-223191 Tocris Cat# 3858 Tetrodotoxin EMD Biosciences Cat# 554412 Critical Commercial Assays cAMP Direct Immunoassay Detection Kit Abcam Cat# ab138880 Experimental Models: Organisms/Strains GF, HM, and CR mice Taconic Farms Swiss Webster 129/Sv WT and 5-HT4R / mice Compan et al., 2004 129/Sv Recombinant DNA pNBU2 Whitaker et al., 2017 N/A Oligonucleotides 5-HT4R Forward: 50-AGTTCCAACGAGGGTTTCAGG-30 Reverse: 50-CAGCAGGTTGCCCAAGATG-30 Bhattarai et al., 2017b N/A Turicibacter Forward: 50-GCGCGCAGGTGGTTAATTAAGTCT-30 Reverse: 50-TCAGTGTCAGTTGCAGACCAGGAA-30 This work N/A Universal Forward: 50-AGAGTTTGATCCTGGCTCAG-30 Reverse: 50-GACGGGCGGTGWGTRCA-30 Bhattarai and Kashyap, 2016 N/A Software and Algorithms ImageJ NIH Version 1.50i GraphPad PRISM GraphPad Software Version 7.0a (Continued on next page) e1 Cell Host & Microbe 23, 775–785.e1–e5, June 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acquire and Analyze Physiologic Instruments Version 2.0 Leica Application Suite X Leica N/A Other Synergy MX plate reader BioTek Gen5 1.09 Confocal live cell microscopy Leica Microsystems DMi8 Ussing Chamber Physiologic Instruments Cat# P2300 Voltage-clamp amplifier Physiologic Instruments Version VCC MC8 LC-MS/MS Waters N/A
Recombinant:Article Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Bacteroides thetaiotaomicron VPI-5482 ATCC ATCC 29148 Bacteroides thetaiotaomicron VPI-5482 pNBU2 This work N/A Bacteroides thetaiotaomicron VPI-5482 pNBU2::trpD This work N/A Chemicals, Peptides, and Recombinant Proteins Ca2+ Mg-free PBS Corning 21-040-CV Gentle cell dissociation media STEMCELL Technologies Cat# 7174 Bovine serum albumin Sigma Life Science Cas# 9048-46-8 DMEM-F/12 media Life Technologies Cat# 11330-032 IntestiCultTM organoid growth media STEMCELL Technologies Cat# 6000 Matrigel Corning Cat# 356231 Calcein green Invitrogen Cat# C3100MP Tryptamine hydrochloride Aldrich Cas# 343-92-2 GR-113808 Tocris Bioscience Cas# 144625-51-4 Ondansetron Sigma-Aldrich Cas# 99614-01-04 Forskolin Sigma-Aldrich Cas# 66575-29-9 Erythromycin Sigma Life Science Cas# 114-07-8 Gentamycin Sigma Life Science Cas# 1405-41-0 Methanol Sigma-Aldrich Cas# 67-56-1 Methylcellulose Sigma-Aldrich Cas#9004-67-5 Carmine red Sigma-Aldrich Cas# 1390-65-4 5-Nitro-2-(3-phenylpropylamino) benzoic acid Sigma-Aldrich Cas# 107254-86-4 Amiloride hydrochloride Sigma-Aldrich Cas# 2016-88-8 CH-223191 Tocris Cat# 3858 Tetrodotoxin EMD Biosciences Cat# 554412 Critical Commercial Assays cAMP Direct Immunoassay Detection Kit Abcam Cat# ab138880 Experimental Models: Organisms/Strains GF, HM, and CR mice Taconic Farms Swiss Webster 129/Sv WT and 5-HT4R / mice Compan et al., 2004 129/Sv Recombinant DNA pNBU2 Whitaker et al., 2017 N/A Oligonucleotides 5-HT4R Forward: 50-AGTTCCAACGAGGGTTTCAGG-30 Reverse: 50-CAGCAGGTTGCCCAAGATG-30 Bhattarai et al., 2017b N/A Turicibacter Forward: 50-GCGCGCAGGTGGTTAATTAAGTCT-30 Reverse: 50-TCAGTGTCAGTTGCAGACCAGGAA-30 This work N/A Universal Forward: 50-AGAGTTTGATCCTGGCTCAG-30 Reverse: 50-GACGGGCGGTGWGTRCA-30 Bhattarai and Kashyap, 2016 N/A Software and Algorithms ImageJ NIH Version 1.50i GraphPad PRISM GraphPad Software Version 7.0a (Continued on next page) e1 Cell Host & Microbe 23, 775–785.e1–e5, June 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acquire and Analyze Physiologic Instruments Version 2.0 Leica Application Suite X Leica N/A Other Synergy MX plate reader BioTek Gen5 1.09 Confocal live cell microscopy Leica Microsystems DMi8 Ussing Chamber Physiologic Instruments Cat# P2300 Voltage-clamp amplifier Physiologic Instruments Version VCC MC8 LC-MS/MS Waters N/A
Gentle:Article Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Bacteroides thetaiotaomicron VPI-5482 ATCC ATCC 29148 Bacteroides thetaiotaomicron VPI-5482 pNBU2 This work N/A Bacteroides thetaiotaomicron VPI-5482 pNBU2::trpD This work N/A Chemicals, Peptides, and Recombinant Proteins Ca2+ Mg-free PBS Corning 21-040-CV Gentle cell dissociation media STEMCELL Technologies Cat# 7174 Bovine serum albumin Sigma Life Science Cas# 9048-46-8 DMEM-F/12 media Life Technologies Cat# 11330-032 IntestiCultTM organoid growth media STEMCELL Technologies Cat# 6000 Matrigel Corning Cat# 356231 Calcein green Invitrogen Cat# C3100MP Tryptamine hydrochloride Aldrich Cas# 343-92-2 GR-113808 Tocris Bioscience Cas# 144625-51-4 Ondansetron Sigma-Aldrich Cas# 99614-01-04 Forskolin Sigma-Aldrich Cas# 66575-29-9 Erythromycin Sigma Life Science Cas# 114-07-8 Gentamycin Sigma Life Science Cas# 1405-41-0 Methanol Sigma-Aldrich Cas# 67-56-1 Methylcellulose Sigma-Aldrich Cas#9004-67-5 Carmine red Sigma-Aldrich Cas# 1390-65-4 5-Nitro-2-(3-phenylpropylamino) benzoic acid Sigma-Aldrich Cas# 107254-86-4 Amiloride hydrochloride Sigma-Aldrich Cas# 2016-88-8 CH-223191 Tocris Cat# 3858 Tetrodotoxin EMD Biosciences Cat# 554412 Critical Commercial Assays cAMP Direct Immunoassay Detection Kit Abcam Cat# ab138880 Experimental Models: Organisms/Strains GF, HM, and CR mice Taconic Farms Swiss Webster 129/Sv WT and 5-HT4R / mice Compan et al., 2004 129/Sv Recombinant DNA pNBU2 Whitaker et al., 2017 N/A Oligonucleotides 5-HT4R Forward: 50-AGTTCCAACGAGGGTTTCAGG-30 Reverse: 50-CAGCAGGTTGCCCAAGATG-30 Bhattarai et al., 2017b N/A Turicibacter Forward: 50-GCGCGCAGGTGGTTAATTAAGTCT-30 Reverse: 50-TCAGTGTCAGTTGCAGACCAGGAA-30 This work N/A Universal Forward: 50-AGAGTTTGATCCTGGCTCAG-30 Reverse: 50-GACGGGCGGTGWGTRCA-30 Bhattarai and Kashyap, 2016 N/A Software and Algorithms ImageJ NIH Version 1.50i GraphPad PRISM GraphPad Software Version 7.0a (Continued on next page) e1 Cell Host & Microbe 23, 775–785.e1–e5, June 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acquire and Analyze Physiologic Instruments Version 2.0 Leica Application Suite X Leica N/A Other Synergy MX plate reader BioTek Gen5 1.09 Confocal live cell microscopy Leica Microsystems DMi8 Ussing Chamber Physiologic Instruments Cat# P2300 Voltage-clamp amplifier Physiologic Instruments Version VCC MC8 LC-MS/MS Waters N/A
Software:Article Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Bacteroides thetaiotaomicron VPI-5482 ATCC ATCC 29148 Bacteroides thetaiotaomicron VPI-5482 pNBU2 This work N/A Bacteroides thetaiotaomicron VPI-5482 pNBU2::trpD This work N/A Chemicals, Peptides, and Recombinant Proteins Ca2+ Mg-free PBS Corning 21-040-CV Gentle cell dissociation media STEMCELL Technologies Cat# 7174 Bovine serum albumin Sigma Life Science Cas# 9048-46-8 DMEM-F/12 media Life Technologies Cat# 11330-032 IntestiCultTM organoid growth media STEMCELL Technologies Cat# 6000 Matrigel Corning Cat# 356231 Calcein green Invitrogen Cat# C3100MP Tryptamine hydrochloride Aldrich Cas# 343-92-2 GR-113808 Tocris Bioscience Cas# 144625-51-4 Ondansetron Sigma-Aldrich Cas# 99614-01-04 Forskolin Sigma-Aldrich Cas# 66575-29-9 Erythromycin Sigma Life Science Cas# 114-07-8 Gentamycin Sigma Life Science Cas# 1405-41-0 Methanol Sigma-Aldrich Cas# 67-56-1 Methylcellulose Sigma-Aldrich Cas#9004-67-5 Carmine red Sigma-Aldrich Cas# 1390-65-4 5-Nitro-2-(3-phenylpropylamino) benzoic acid Sigma-Aldrich Cas# 107254-86-4 Amiloride hydrochloride Sigma-Aldrich Cas# 2016-88-8 CH-223191 Tocris Cat# 3858 Tetrodotoxin EMD Biosciences Cat# 554412 Critical Commercial Assays cAMP Direct Immunoassay Detection Kit Abcam Cat# ab138880 Experimental Models: Organisms/Strains GF, HM, and CR mice Taconic Farms Swiss Webster 129/Sv WT and 5-HT4R / mice Compan et al., 2004 129/Sv Recombinant DNA pNBU2 Whitaker et al., 2017 N/A Oligonucleotides 5-HT4R Forward: 50-AGTTCCAACGAGGGTTTCAGG-30 Reverse: 50-CAGCAGGTTGCCCAAGATG-30 Bhattarai et al., 2017b N/A Turicibacter Forward: 50-GCGCGCAGGTGGTTAATTAAGTCT-30 Reverse: 50-TCAGTGTCAGTTGCAGACCAGGAA-30 This work N/A Universal Forward: 50-AGAGTTTGATCCTGGCTCAG-30 Reverse: 50-GACGGGCGGTGWGTRCA-30 Bhattarai and Kashyap, 2016 N/A Software and Algorithms ImageJ NIH Version 1.50i GraphPad PRISM GraphPad Software Version 7.0a (Continued on next page) e1 Cell Host & Microbe 23, 775–785.e1–e5, June 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acquire and Analyze Physiologic Instruments Version 2.0 Leica Application Suite X Leica N/A Other Synergy MX plate reader BioTek Gen5 1.09 Confocal live cell microscopy Leica Microsystems DMi8 Ussing Chamber Physiologic Instruments Cat# P2300 Voltage-clamp amplifier Physiologic Instruments Version VCC MC8 LC-MS/MS Waters N/A
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